بررسی تغییرات بیانmiR-101و ارتباط آن با میزان بیان ژن‌های سرکوب‌کننده تومور PTENو MAGI2در سه رده سلولی سرطان پروستات

Introduction: Defects in PTEN gene play an important role in the initiation of the prostate cancer. MAGI2 is recognized as a frame which assists in stabilizing PTEN protein and enhances its tumor suppressor function. As miR-101 down-regulates MAGI2 expression, it can indirectly cause to reduce the PTEN activity. Methods:LnCap, PC3 and DU-145 prostate cancer cell lines were studied in order to investigate expression ofPTEN,MAGI2and miR-101 using TaqMan probe Real-time PCR assay. The relative expression of each gene was determined based on their CT value and CT method. Results:The study results demonstrated that miR-101 and MAGI2 expression was down-regulated in all the three cell lines in comparison to prostate cancer tissue. However, despite the high expression of miR-101 in prostate cancer tissue, MAGI2 expression was not significantly affected in this sample. The expression of PTEN gene in LnCap and PC3 cells was significantly lower than its expression in DU-145 cells. This can be explained by the complete loss of the PTEN gene at DNA level. Conclusion: The findings of the present study confirmed the loss of the expression of PTEN and MAGI2 genes in PC3 and LnCap cells, which may be due to MAGI2gene rearrangements in a mutually exclusive manner.